| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This SHH Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SHH |
| Gene ID: |
6469 |
| Uniprot ID: |
SHH_HUMAN |
| Specificity: |
This kit recognizes Human SHH in samples. No significant cross-reactivity or interference between Human SHH and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum. Sonic hedgehog protein N-product: The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO. |
| Protein Name | Sonic Hedgehog ProteinShhHhg-1Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing DomainsShhnc Cleaved Into - Sonic Hedgehog Protein N-ProductShhnShh N-Terminal Processed Signaling DomainsShhnp |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-5358346Reactome: R-HSA-5362768Reactome: R-HSA-5362798Reactome: R-HSA-5632681Reactome: R-HSA-5632684Reactome: R-HSA-5635838Reactome: R-HSA-5658034Reactome: R-HSA-9758920Reactome: R-HSA-9796292 |
| Cellular Localisation | Sonic Hedgehog Protein: Endoplasmic Reticulum MembraneGolgi Apparatus MembraneSecretedCo-Localizes With Hhat In The Er And Golgi MembraneSonic Hedgehog Protein N-Product: Cell MembraneLipid-AnchorThe Dual-Lipidated Sonic Hedgehog Protein N-Product (Shhnp) Is Firmly Tethered To The Cell Membrane Where It Forms MultimersFurther Solubilization And Release From The Cell Surface Seem To Be Achieved Through Different MechanismsIncluding The Interaction With Disp1 And Scube2Movement By Lipoprotein ParticlesTransport By Cellular Extensions Called Cytonemes Or By The Proteolytic Removal Of Both Terminal Lipidated Peptides |
| Alternative ELISA Names | Sonic Hedgehog Protein ELISA kitShh ELISA kitHhg-1 ELISA kitShh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains ELISA kitShhnc Cleaved Into - Sonic Hedgehog Protein N-Product ELISA kitShhn ELISA kitShh N-Terminal Processed Signaling Domains ELISA kitShhnp ELISA kitSHH ELISA kit |
| Specificity | This kit recognizes Human SHH in samples. No significant cross-reactivity or interference between Human SHH and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 94 |
| EDTA plasma (n=8) | 94-107 | 101 |
| Cell culture media (n=8) | 87-98 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 265.23 | 629.21 | 1862.20 | 280.01 | 601.38 | 1709.28 |
| Standard deviation | 13.39 | 32.09 | 59.40 | 15.57 | 26.52 | 68.03 |
| CV (%) | 5.05 | 5.10 | 3.19 | 5.56 | 4.41 | 3.98 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
