| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This human SFRP2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
SFRP2 |
| Gene ID: |
6423 |
| Uniprot ID: |
SFRP2_HUMAN |
| Specificity: |
This kit recognizes Human SFRP2 in samples. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. |
| Function | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. |
| Protein Name | Secreted Frizzled-Related Protein 2Frp-2Sfrp-2Secreted Apoptosis-Related Protein 1Sarp-1 |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3772470 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Secreted Frizzled-Related Protein 2 CLIA kitFrp-2 CLIA kitSfrp-2 CLIA kitSecreted Apoptosis-Related Protein 1 CLIA kitSarp-1 CLIA kitSFRP2 CLIA kitFRP2 CLIA kitSARP1 CLIA kitFKSG12 CLIA kitUNQ361 CLIA kitPRO697 CLIA kit |
| Specificity | This kit recognizes Human SFRP2 in samples. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 83-98 | 90 |
| EDTA plasma(n=8) | 100-117 | 108 |
| Cell culture media(n=8) | 86-99 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.00 | 1.92 | 6.77 | 0.99 | 1.81 | 6.55 |
| Standard deviation | 0.11 | 0.17 | 0.73 | 0.09 | 0.13 | 0.63 |
| CV (%) | 11.00 | 8.85 | 10.78 | 9.09 | 7.18 | 9.62 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
