| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.055ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This SESN2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
SESN2 |
| Gene ID: |
83667 |
| Uniprot ID: |
SESN2_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated by ULK1 at multiple sites. Ubiquitinated at Lys-175 by RNF167 via 'Lys-63'-linked polyubiquitination in response to leucine deprivation: ubiquitination promotes SESN2-interaction with the GATOR2 complex, leading to inhibit the TORC1 signaling pathway. Deubiquitinated at Lys-175 by STAMBPL1, promoting the TORC1 signaling pathway. Ubiquitinated by RNF186.ubiquitination mediates proteasomal degradation. |
| Function | Functions as an intracellular leucine sensor that negatively regulates the mTORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents mTORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via mTORC1. May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress. Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein. Was originally reported to contribute to oxidative stress resistance by reducing PRDX1. However, this could not be confirmed. |
| Protein Name | Sestrin-2Hypoxia-Induced Gene |
| Database Links | Reactome: R-HSA-5628897Reactome: R-HSA-9639288Reactome: R-HSA-9755511 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Sestrin-2 ELISA kitHypoxia-Induced Gene ELISA kitSESN2 ELISA kitHi95 ELISA kitSEST2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
