| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This SEMA4D Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SEMA4D |
| Gene ID: |
10507 |
| Uniprot ID: |
SEM4D_HUMAN |
| Specificity: |
This kit recognizes Human SEMA4D in samples. No significant cross-reactivity or interference between Human SEMA4D and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system.induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. |
| Protein Name | Semaphorin-4dA8Bb18Gr3Cd Antigen Cd100 |
| Database Links | Reactome: R-HSA-416550Reactome: R-HSA-416572Reactome: R-HSA-416700 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Semaphorin-4d ELISA kitA8 ELISA kitBb18 ELISA kitGr3 ELISA kitCd Antigen Cd100 ELISA kitSEMA4D ELISA kitC9orf164 ELISA kitCD100 ELISA kitSEMAJ ELISA kit |
| Specificity | This kit recognizes Human SEMA4D in samples. No significant cross-reactivity or interference between Human SEMA4D and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-107 | 98 |
| EDTA plasma (n=8) | 84-98 | 91 |
| Cell culture media (n=8) | 94-105 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.05 | 2.48 | 9.92 | 0.96 | 2.31 | 10.06 |
| Standard deviation | 0.07 | 0.14 | 0.46 | 0.06 | 0.10 | 0.45 |
| CV (%) | 6.67 | 5.65 | 4.64 | 6.25 | 4.33 | 4.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
