| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This SELENOP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
SELENOP |
| Gene ID: |
6414 |
| Uniprot ID: |
SEPP1_HUMAN |
| Specificity: |
This kit recognizes Human SEPP1 in samples. No significant cross-reactivity or interference between Human SEPP1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation sites are present in the extracellular medium. |
| Function | Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. |
| Protein Name | Selenoprotein PSep |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | SecretedPasses From Plasma Into The Glomerular Filtrate Where It Is Removed By Endocytosis Mediated By Lrp2 In The Proximal Tubule Epithelium |
| Alternative ELISA Names | Selenoprotein P ELISA kitSep ELISA kitSELENOP ELISA kitSELP ELISA kitSEPP1 ELISA kit |
| Specificity | This kit recognizes Human SEPP1 in samples. No significant cross-reactivity or interference between Human SEPP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-100 | 93 |
| EDTA plasma (n=8) | 95-109 | 100 |
| Cell culture media (n=8) | 87-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.48 | 1.35 | 3.52 | 0.51 | 1.38 | 3.78 |
| Standard deviation | 0.03 | 0.06 | 0.12 | 0.03 | 0.07 | 0.20 |
| CV (%) | 6.25 | 4.44 | 3.41 | 5.88 | 5.07 | 5.29 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
