• Human SDF4 (45 kDa calcium-binding protein) Sandwich ELISA (STJE0002992)

Human SDF4 (45 kDa calcium-binding protein) Sandwich ELISA (STJE0002992)

SKU:
STJE0002992

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This SDF4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SDF4
Gene ID: 51150
Uniprot ID: CAB45_HUMAN
Specificity: This kit recognizes Human SDF-4 in samples. No significant cross-reactivity or interference between Human SDF-4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini.
Protein Name 45 Kda Calcium-Binding Protein
Cab45
Stromal Cell-Derived Factor 4
Sdf-4
Cellular Localisation Isoform 1: Golgi Apparatus Lumen
Isoform 5: Cytoplasm
Cell Membrane
Cell Projection
Bleb
Isoform 5 Colocalizes With Stx3 And Stxbp1 Isoform 2 At The Plasma Membrane And Cell Surface Blebs
Alternative ELISA Names 45 Kda Calcium-Binding Protein ELISA kit
Cab45 ELISA kit
Stromal Cell-Derived Factor 4 ELISA kit
Sdf-4 ELISA kit
SDF4 ELISA kit
CAB45 ELISA kit
PSEC0034 ELISA kit
Specificity This kit recognizes Human SDF-4 in samples. No significant cross-reactivity or interference between Human SDF-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-100 94
EDTA plasma (n=8) 90-101 95
Cell culture media (n=8) 89-103 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 106.01 228.75 818.96 109.06 210.34 780.44
Standard deviation 5.34 12.63 44.80 5.66 10.66 26.85
CV (%) 5.04 5.52 5.47 5.19 5.07 3.44
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance