• Human SCUBE1 (Signal peptide, CUB and EGF-like domain-containing protein 1) Sandwich ELISA (STJE0003819)

Human SCUBE1 (Signal peptide, CUB and EGF-like domain-containing protein 1) Sandwich ELISA (STJE0003819)

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STJE0003819

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description: This SCUBE1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SCUBE1
Gene ID: 80274
Uniprot ID: SCUB1_HUMAN
Specificity: This kit recognizes Human SCUBE1 in samples. No significant cross-reactivity or interference between Human SCUBE1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated. Could be proteolytically cleaved to release a smaller active fragment.
Function Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
Protein Name Signal Peptide - Cub And Egf-Like Domain-Containing Protein 1
Database Links Reactome: R-HSA-1474228
Cellular Localisation Secreted
Cell Membrane
Peripheral Membrane Protein
Alternative ELISA Names Signal Peptide - Cub And Egf-Like Domain-Containing Protein 1 ELISA kit
SCUBE1 ELISA kit
Specificity This kit recognizes Human SCUBE1 in samples. No significant cross-reactivity or interference between Human SCUBE1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-95 90
EDTA plasma (n=8) 95-109 101
Cell culture media (n=8) 83-95 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.00 4.25 18.34 1.83 4.51 17.72
Standard deviation 0.11 0.25 0.97 0.11 0.21 0.70
CV (%) 5.50 5.88 5.29 6.01 4.66 3.95
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance