| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This S100A12 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
S100A12 |
| Gene ID: |
6283 |
| Uniprot ID: |
S10AC_HUMAN |
| Specificity: |
This kit recognizes Human S100A12 in samples. No significant cross-reactivity or interference between Human S100A12 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases.MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. |
| Protein Name | Protein S100-A12CgrpCalcium-Binding Protein In Amniotic Fluid 1Caaf1Calgranulin-CCagcExtracellular Newly Identified Rage-Binding ProteinEn-RageMigration Inhibitory Factor-Related Protein 6Mrp-6P6Neutrophil S100 ProteinS100 Calcium-Binding Protein A12 Cleaved Into - Calcitermin |
| Database Links | Reactome: R-HSA-445989Reactome: R-HSA-6798695Reactome: R-HSA-879415Reactome: R-HSA-933542 |
| Cellular Localisation | SecretedCytoplasmCytoskeletonCell MembranePeripheral Membrane ProteinPredominantly Localized In The CytoplasmUpon Elevation Of The Intracellular Calcium LevelTranslocated From The Cytoplasm To The Cytoskeleton And The Cell MembraneUpon Neutrophil Activation Is Secreted Via A Microtubule-MediatedAlternative Pathway |
| Alternative ELISA Names | Protein S100-A12 ELISA kitCgrp ELISA kitCalcium-Binding Protein In Amniotic Fluid 1 ELISA kitCaaf1 ELISA kitCalgranulin-C ELISA kitCagc ELISA kitExtracellular Newly Identified Rage-Binding Protein ELISA kitEn-Rage ELISA kitMigration Inhibitory Factor-Related Protein 6 ELISA kitMrp-6 ELISA kitP6 ELISA kitNeutrophil S100 Protein ELISA kitS100 Calcium-Binding Protein A12 Cleaved Into - Calcitermin ELISA kitS100A12 ELISA kit |
| Specificity | This kit recognizes Human S100A12 in samples. No significant cross-reactivity or interference between Human S100A12 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 98 |
| EDTA plasma (n=8) | 91-107 | 98 |
| Cell culture media (n=8) | 89-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.49 | 1.08 | 4.12 | 0.48 | 1.06 | 3.86 |
| Standard deviation | 0.03 | 0.05 | 0.14 | 0.03 | 0.05 | 0.15 |
| CV (%) | 6.12 | 4.63 | 3.40 | 6.25 | 4.72 | 3.89 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
