| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.116ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This RSPO3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
RSPO3 |
| Gene ID: |
84870 |
| Uniprot ID: |
RSPO3_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node. Highly expressed in endothelial cells. |
| Post Translational Modifications | |
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3. |
| Protein Name | R-Spondin-3Protein With Tsp Type-1 RepeatHpwtsrRoof Plate-Specific Spondin-3Hrspo3Thrombospondin Type-1 Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-4641263Reactome: R-HSA-8939256 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | R-Spondin-3 ELISA kitProtein With Tsp Type-1 Repeat ELISA kitHpwtsr ELISA kitRoof Plate-Specific Spondin-3 ELISA kitHrspo3 ELISA kitThrombospondin Type-1 Domain-Containing Protein 2 ELISA kitRSPO3 ELISA kitPWTSR ELISA kitTHSD2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
