| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
7.3pg/mL |
| Detection Limit: |
15.6-1000pg/mL |
| Short Description: |
This RNASEL Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
RNASEL |
| Gene ID: |
6041 |
| Uniprot ID: |
RN5A_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Highly expressed in spleen and thymus followed by prostate, testis, uterus, small intestine, colon and peripheral blood leukocytes. |
| Post Translational Modifications | |
| Function | Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover. Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length. |
| Protein Name | 2-5a-Dependent Ribonuclease2-5a-Dependent RnaseRibonuclease 4Ribonuclease LRnase L |
| Database Links | Reactome: R-HSA-8983711Reactome: R-HSA-909733 |
| Cellular Localisation | CytoplasmMitochondrion |
| Alternative ELISA Names | 2-5a-Dependent Ribonuclease ELISA kit2-5a-Dependent Rnase ELISA kitRibonuclease 4 ELISA kitRibonuclease L ELISA kitRnase L ELISA kitRNASEL ELISA kitRNS4 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
