Applications: |
ELISA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
0.38ng/mL |
Detection Limit: |
0.63~40ng/mL |
Short Description: |
This RNASE2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Colormetric |
Gene Symbol: |
RNASE2 |
Gene ID: |
6036 |
Uniprot ID: |
RNAS2_HUMAN |
Specificity: |
This kit recognizes Human EDN in samples. No significant cross-reactivity or interference between Human EDN and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Post Translational Modifications | A particular signal processing and glycosylation pattern may differentiate the UpI2 RNase, found specifically in pregnant women urine, from other nonsecretory RNases. |
Function | This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities. |
Protein Name | Non-Secretory RibonucleaseEosinophil-Derived NeurotoxinRnase Upi-2Ribonuclease 2Rnase 2Ribonuclease Us |
Database Links | Reactome: R-HSA-6798695 |
Cellular Localisation | LysosomeCytoplasmic GranuleMatrix Of Eosinophil's Large Specific Granule |
Alternative ELISA Names | Non-Secretory Ribonuclease ELISA kitEosinophil-Derived Neurotoxin ELISA kitRnase Upi-2 ELISA kitRibonuclease 2 ELISA kitRnase 2 ELISA kitRibonuclease Us ELISA kitRNASE2 ELISA kitEDN ELISA kitRNS2 ELISA kit |
Specificity | This kit recognizes Human EDN in samples. No significant cross-reactivity or interference between Human EDN and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 88-103 | 95 |
EDTA plasma (n=8) | 85-99 | 91 |
Cell culture media (n=8) | 83-95 | 90 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 1.91 | 5.96 | 17.11 | 1.73 | 5.46 | 18.77 |
Standard deviation | 0.12 | 0.25 | 0.63 | 0.09 | 0.26 | 0.68 |
CV (%) | 6.28 | 4.19 | 3.68 | 5.20 | 4.76 | 3.62 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance