| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human RETN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
RETN |
| Gene ID: |
56729 |
| Uniprot ID: |
RETN_HUMAN |
| Specificity: |
This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in white adipose tissue (at protein level). Widely expressed, with particularly strong expression in lung, bone marrow, breast and peripheral blood. Expressed strongly in bone marrow and at lower levels in lung, but not detected in other tissues. Isoform 2 is detected in adipose tissue, bone marrow, brain, lung, peripheral blood, placenta and thymus. |
| Function | Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Promotes chemotaxis in myeloid cells. |
| Protein Name | ResistinAdipose Tissue-Specific Secretory FactorAdsfC/Ebp-Epsilon-Regulated Myeloid-Specific Secreted Cysteine-Rich ProteinCysteine-Rich Secreted Protein A12-Alpha-Like 2Cysteine-Rich Secreted Protein Fizz3 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-9615017 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Resistin CLIA kitAdipose Tissue-Specific Secretory Factor CLIA kitAdsf CLIA kitC/Ebp-Epsilon-Regulated Myeloid-Specific Secreted Cysteine-Rich Protein CLIA kitCysteine-Rich Secreted Protein A12-Alpha-Like 2 CLIA kitCysteine-Rich Secreted Protein Fizz3 CLIA kitRETN CLIA kitFIZZ3 CLIA kitHXCP1 CLIA kitRSTN CLIA kitUNQ407 CLIA kitPRO1199 CLIA kit |
| Specificity | This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-105 | 96 |
| EDTA plasma(n=8) | 97-113 | 104 |
| Cell culture media(n=8) | 86-100 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 43.23 | 66.99 | 375.61 | 45.42 | 64.95 | 344.70 |
| Standard deviation | 5.26 | 7.31 | 24.64 | 4.88 | 7.20 | 38.78 |
| CV (%) | 12.17 | 10.91 | 6.56 | 10.74 | 11.09 | 11.25 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
