• Human RETN (Resistin) CLIA Kit (STJC0000554)

Human RETN (Resistin) CLIA Kit (STJC0000554)

SKU:
STJC0000554

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human RETN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: RETN
Gene ID: 56729
Uniprot ID: RETN_HUMAN
Specificity: This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in white adipose tissue (at protein level). Widely expressed, with particularly strong expression in lung, bone marrow, breast and peripheral blood. Expressed strongly in bone marrow and at lower levels in lung, but not detected in other tissues. Isoform 2 is detected in adipose tissue, bone marrow, brain, lung, peripheral blood, placenta and thymus.
Function Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Promotes chemotaxis in myeloid cells.
Protein Name Resistin
Adipose Tissue-Specific Secretory Factor
Adsf
C/Ebp-Epsilon-Regulated Myeloid-Specific Secreted Cysteine-Rich Protein
Cysteine-Rich Secreted Protein A12-Alpha-Like 2
Cysteine-Rich Secreted Protein Fizz3
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-9615017
Cellular Localisation Secreted
Alternative CLIA Names Resistin CLIA kit
Adipose Tissue-Specific Secretory Factor CLIA kit
Adsf CLIA kit
C/Ebp-Epsilon-Regulated Myeloid-Specific Secreted Cysteine-Rich Protein CLIA kit
Cysteine-Rich Secreted Protein A12-Alpha-Like 2 CLIA kit
Cysteine-Rich Secreted Protein Fizz3 CLIA kit
RETN CLIA kit
FIZZ3 CLIA kit
HXCP1 CLIA kit
RSTN CLIA kit
UNQ407 CLIA kit
PRO1199 CLIA kit
Specificity This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-105 96
EDTA plasma(n=8) 97-113 104
Cell culture media(n=8) 86-100 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 43.23 66.99 375.61 45.42 64.95 344.70
Standard deviation 5.26 7.31 24.64 4.88 7.20 38.78
CV (%) 12.17 10.91 6.56 10.74 11.09 11.25
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance