| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94pg/mL |
| Detection Limit: |
1.56~100pg/mL |
| Short Description: |
This RET Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
RET |
| Gene ID: |
5979 |
| Uniprot ID: |
RET_HUMAN |
| Specificity: |
This kit recognizes Human RET in samples. No significant cross-reactivity or interference between Human RET and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Post Translational Modifications | Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons. |
| Function | Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis.involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor.in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers.these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways. Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL. |
| Protein Name | Proto-Oncogene Tyrosine-Protein Kinase Receptor RetCadherin Family Member 12Proto-Oncogene C-Ret Cleaved Into - Soluble Ret Kinase Fragment - Extracellular Cell-Membrane Anchored Ret Cadherin 120 Kda Fragment |
| Database Links | Reactome: R-HSA-5673001Reactome: R-HSA-8853659Reactome: R-HSA-9768919 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndosome MembranePredominantly Located On The Plasma MembraneIn The Presence Of Sorl1 And Gfra1Directed To Endosomes |
| Alternative ELISA Names | Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret ELISA kitCadherin Family Member 12 ELISA kitProto-Oncogene C-Ret Cleaved Into - Soluble Ret Kinase Fragment - Extracellular Cell-Membrane Anchored Ret Cadherin 120 Kda Fragment ELISA kitRET ELISA kitCDHF12 ELISA kitCDHR16 ELISA kitPTC ELISA kitRET51 ELISA kit |
| Specificity | This kit recognizes Human RET in samples. No significant cross-reactivity or interference between Human RET and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-102 | 95 |
| EDTA plasma (n=8) | 89-101 | 94 |
| Cell culture media (n=8) | 92-104 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 4.92 | 13.44 | 48.09 | 4.58 | 14.53 | 43.55 |
| Standard deviation | 0.28 | 0.64 | 2.14 | 0.30 | 0.62 | 1.65 |
| CV (%) | 5.69 | 4.74 | 4.46 | 6.60 | 4.24 | 3.79 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
