| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human REG1A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
REG1A |
| Gene ID: |
5967 |
| Uniprot ID: |
REG1A_HUMAN |
| Specificity: |
This kit recognizes Human REG1 Alpha in samples. No significant cross-reactivity or interference between Human REG1 Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients. |
| Post Translational Modifications | The composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine. |
| Function | Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. |
| Protein Name | Lithostathine-1-AlphaIslet Cells Regeneration FactorIcrfIslet Of Langerhans Regenerating ProteinRegPancreatic Stone ProteinPspPancreatic Thread ProteinPtpRegenerating Islet-Derived Protein 1-AlphaReg-1-AlphaRegenerating Protein I Alpha |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Lithostathine-1-Alpha CLIA kitIslet Cells Regeneration Factor CLIA kitIcrf CLIA kitIslet Of Langerhans Regenerating Protein CLIA kitReg CLIA kitPancreatic Stone Protein CLIA kitPsp CLIA kitPancreatic Thread Protein CLIA kitPtp CLIA kitRegenerating Islet-Derived Protein 1-Alpha CLIA kitReg-1-Alpha CLIA kitRegenerating Protein I Alpha CLIA kitREG1A CLIA kitPSPS CLIA kitPSPS1 CLIA kitREG CLIA kit |
| Specificity | This kit recognizes Human REG1α in samples. No significant cross-reactivity or interference between Human REG1α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 101-115 | 107 |
| EDTA plasma(n=8) | 98-111 | 104 |
| Cell culture media(n=8) | 93-107 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 49.53 | 145.89 | 463.35 | 49.34 | 151.98 | 478.89 |
| Standard deviation | 5.90 | 12.62 | 40.17 | 4.01 | 16.78 | 37.88 |
| CV (%) | 11.91 | 8.65 | 8.67 | 8.13 | 11.04 | 7.91 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
