| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.057ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This RAD50 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | RAD50 |
| Gene ID: | 10111 |
| Uniprot ID: | RAD50_HUMAN |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. |
| Post Translational Modifications | |
| Function | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. |
| Protein Name | Dna Repair Protein Rad50Hrad50 |
| Database Links | Reactome: R-HSA-2559586Reactome: R-HSA-5685938Reactome: R-HSA-5685939Reactome: R-HSA-5685942Reactome: R-HSA-5693548Reactome: R-HSA-5693554Reactome: R-HSA-5693565Reactome: R-HSA-5693568Reactome: R-HSA-5693571Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-912446Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusChromosomeTelomereLocalizes To Discrete Nuclear Foci After Treatment With Genotoxic Agents |
| Alternative ELISA Names | Dna Repair Protein Rad50 ELISA kitHrad50 ELISA kitRAD50 ELISA kit |
| output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
