| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human PTN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PTN |
| Gene ID: |
5764 |
| Uniprot ID: |
PTN_HUMAN |
| Specificity: |
This kit recognizes Human PTN in samples. No significant cross-reactivity or interference between Human PTN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Osteoblast and brain. |
| Post Translational Modifications | Phosphorylated by NEK6. |
| Function | Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK, CTNNB1 or AFAP1L2 in order to activate the PI3K-AKT pathway. Through PTPRZ1 binding controls oligodendrocyte precursor cell differentiation by enhancing the phosphorylation of AFAP1L2 in order to activate the PI3K-AKT pathway. Forms a complex with PTPRZ1 and integrin alpha-V/beta-3 (ITGAV:ITGB3) that stimulates endothelial cell migration through SRC dephosphorylation and activation that consequently leads to ITGB3 'Tyr-773' phosphorylation. In adult hippocampus promotes dendritic arborization, spine development, and functional integration and connectivity of newborn granule neurons through ALK by activating AKT signaling pathway. Binds GPC2 and chondroitin sulfate proteoglycans (CSPGs) at the neuron surface, leading to abrogation of binding between PTPRS and CSPGs and neurite outgrowth promotion. Binds SDC3 and mediates bone formation by recruiting and attaching osteoblasts/osteoblast precursors to the sites for new bone deposition. Binds ALK and promotes cell survival and cell proliferation through MAPK pathway activation. Inhibits proliferation and enhances differentiation of neural stem cells by inhibiting FGF2-induced fibroblast growth factor receptor signaling pathway. Mediates regulatory mechanisms in normal hemostasis and in hematopoietic regeneration and in maintaining the balance of myeloid and lymphoid regeneration. In addition may play a role in the female reproductive system, auditory response and the progesterone-induced decidualization pathway. |
| Protein Name | PleiotrophinPtnHeparin-Binding Brain MitogenHbbmHeparin-Binding Growth Factor 8Hbgf-8Heparin-Binding Growth-Associated MoleculeHb-GamHeparin-Binding Neurite Outgrowth-Promoting FactorHbnfHeparin-Binding Neurite Outgrowth-Promoting Factor 1Hbnf-1Osteoblast-Specific Factor 1Osf-1 |
| Database Links | Reactome: R-HSA-201556 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Pleiotrophin CLIA kitPtn CLIA kitHeparin-Binding Brain Mitogen CLIA kitHbbm CLIA kitHeparin-Binding Growth Factor 8 CLIA kitHbgf-8 CLIA kitHeparin-Binding Growth-Associated Molecule CLIA kitHb-Gam CLIA kitHeparin-Binding Neurite Outgrowth-Promoting Factor CLIA kitHbnf CLIA kitHeparin-Binding Neurite Outgrowth-Promoting Factor 1 CLIA kitHbnf-1 CLIA kitOsteoblast-Specific Factor 1 CLIA kitOsf-1 CLIA kitPTN CLIA kitHBNF1 CLIA kitNEGF1 CLIA kit |
| Specificity | This kit recognizes Human PTN in samples. No significant cross-reactivity or interference between Human PTN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-111 | 105 |
| EDTA plasma(n=8) | 95-109 | 101 |
| Cell culture media(n=8) | 99-116 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 42.55 | 69.45 | 363.20 | 46.61 | 70.67 | 395.76 |
| Standard deviation | 4.66 | 6.20 | 29.78 | 5.50 | 6.83 | 45.39 |
| CV (%) | 10.95 | 8.93 | 8.20 | 11.80 | 9.66 | 11.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
