| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This PTHLH Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
PTHLH |
| Gene ID: |
5744 |
| Uniprot ID: |
PTHR_HUMAN |
| Specificity: |
This kit recognizes Human PTHrP in samples. No significant cross-reactivity or interference between Human PTHrP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | There are 3 principal secretory forms, called PTHrP1-36, PTHrP38-94, and osteostatin (PTHrP107-139) arising from endoproteolytic cleavage of the initial translation product. Each of these secretory forms is believed to have one or more of its own receptors that mediates the normal paracrine, autocrine and endocrine actions. |
| Function | Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Up-regulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath. Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1. Osteostatin is a potent inhibitor of osteoclastic bone resorption. |
| Protein Name | Parathyroid Hormone-Related ProteinPth-RpPthrpParathyroid Hormone-Like ProteinPlp Cleaved Into - Pthrp1-36 - Pthrp38-94 - OsteostatinPthrp107-139 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-418555 |
| Cellular Localisation | CytoplasmNucleusSecreted |
| Alternative ELISA Names | Parathyroid Hormone-Related Protein ELISA kitPth-Rp ELISA kitPthrp ELISA kitParathyroid Hormone-Like Protein ELISA kitPlp Cleaved Into - Pthrp1-36 - Pthrp38-94 - Osteostatin ELISA kitPthrp107-139 ELISA kitPTHLH ELISA kitPTHRP ELISA kit |
| Specificity | This kit recognizes Human PTHrP in samples. No significant cross-reactivity or interference between Human PTHrP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-106 | 97 |
| EDTA plasma (n=8) | 94-106 | 99 |
| Cell culture media (n=8) | 87-101 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 52.65 | 129.26 | 465.14 | 51.08 | 133.97 | 507.77 |
| Standard deviation | 3.54 | 5.48 | 22.75 | 2.57 | 6.74 | 25.69 |
| CV (%) | 6.72 | 4.24 | 4.89 | 5.03 | 5.03 | 5.06 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
