| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This PSAP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
PSAP |
| Gene ID: |
5660 |
| Uniprot ID: |
SAP_HUMAN |
| Specificity: |
This kit recognizes Human PSAP in samples. No significant cross-reactivity or interference between Human PSAP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase and galactosylceramide by beta-galactosylceramidase. Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A, GM1 gangliosides by beta-galactosidase and globotriaosylceramide by alpha-galactosidase A. Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases. Saposin-D is a specific sphingomyelin phosphodiesterase activator. Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling. Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases. |
| Protein Name | ProsaposinProactivator Polypeptide Cleaved Into - Saposin-AProtein A - Saposin-B-Val - Saposin-BCerebroside Sulfate ActivatorCsactDispersinSphingolipid Activator Protein 1Sap-1Sulfatide/Gm1 Activator - Saposin-CA1 ActivatorCo-Beta-GlucosidaseGlucosylceramidase ActivatorSphingolipid Activator Protein 2Sap-2 - Saposin-DComponent CProtein C |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1660662Reactome: R-HSA-375276Reactome: R-HSA-418594Reactome: R-HSA-6798695 |
| Cellular Localisation | LysosomeProsaposin: SecretedSecreted As A Fully Glycosylated 70 Kda Protein Composed Of Complex Glycans |
| Alternative ELISA Names | Prosaposin ELISA kitProactivator Polypeptide Cleaved Into - Saposin-A ELISA kitProtein A - Saposin-B-Val - Saposin-B ELISA kitCerebroside Sulfate Activator ELISA kitCsact ELISA kitDispersin ELISA kitSphingolipid Activator Protein 1 ELISA kitSap-1 ELISA kitSulfatide/Gm1 Activator - Saposin-C ELISA kitA1 Activator ELISA kitCo-Beta-Glucosidase ELISA kitGlucosylceramidase Activator ELISA kitSphingolipid Activator Protein 2 ELISA kitSap-2 - Saposin-D ELISA kitComponent C ELISA kitProtein C ELISA kitPSAP ELISA kitGLBA ELISA kitSAP1 ELISA kit |
| Specificity | This kit recognizes Human PSAP in samples. No significant cross-reactivity or interference between Human PSAP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-97 | 92 |
| EDTA plasma (n=8) | 90-102 | 96 |
| Cell culture media (n=8) | 86-101 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.43 | 4.69 | 0.43 | 1.46 | 4.65 |
| Standard deviation | 0.03 | 0.06 | 0.16 | 0.03 | 0.08 | 0.17 |
| CV (%) | 6.52 | 4.20 | 3.41 | 6.98 | 5.48 | 3.66 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
