| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human PROKR2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PROKR2 |
| Gene ID: |
128674 |
| Uniprot ID: |
PKR2_HUMAN |
| Specificity: |
This kit recognizes Human PKR2 in samples. No significant cross-reactivity or interference between Human PKR2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the ileocecum, thyroid gland, pituitary gland, salivary gland, adrenal gland, testis, ovary and brain. |
| Function | Receptor for prokineticin 2. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. |
| Protein Name | Prokineticin Receptor 2Pk-R2G-Protein Coupled Receptor 73-Like 1G-Protein Coupled Receptor I5eGpr73bGprg2 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-416476 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | Prokineticin Receptor 2 CLIA kitPk-R2 CLIA kitG-Protein Coupled Receptor 73-Like 1 CLIA kitG-Protein Coupled Receptor I5e CLIA kitGpr73b CLIA kitGprg2 CLIA kitPROKR2 CLIA kitGPR73L1 CLIA kitPKR2 CLIA kit |
| Specificity | This kit recognizes Human PKR2 in samples. No significant cross-reactivity or interference between Human PKR2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-100 | 91 |
| EDTA plasma(n=8) | 96-110 | 102 |
| Cell culture media(n=8) | 97-112 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 211.34 | 605.70 | 1956.08 | 197.61 | 600.14 | 1932.79 |
| Standard deviation | 22.00 | 70.56 | 167.05 | 16.42 | 61.09 | 193.67 |
| CV (%) | 10.41 | 11.65 | 8.54 | 8.31 | 10.18 | 10.02 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
