| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This PROC Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
PROC |
| Gene ID: |
5624 |
| Uniprot ID: |
PROC_HUMAN |
| Specificity: |
This kit recognizes Human PROC in samples. No significant cross-reactivity or interference between Human PROC and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium. N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. |
| Function | Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function. |
| Protein Name | Vitamin K-Dependent Protein CAnticoagulant Protein CAutoprothrombin IiaBlood Coagulation Factor Xiv Cleaved Into - Vitamin K-Dependent Protein C Light Chain - Vitamin K-Dependent Protein C Heavy Chain - Activation Peptide |
| Database Links | Reactome: R-HSA-140837Reactome: R-HSA-140875Reactome: R-HSA-159740Reactome: R-HSA-159763Reactome: R-HSA-159782Reactome: R-HSA-202733Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedGolgi ApparatusEndoplasmic Reticulum |
| Alternative ELISA Names | Vitamin K-Dependent Protein C ELISA kitAnticoagulant Protein C ELISA kitAutoprothrombin Iia ELISA kitBlood Coagulation Factor Xiv Cleaved Into - Vitamin K-Dependent Protein C Light Chain - Vitamin K-Dependent Protein C Heavy Chain - Activation Peptide ELISA kitPROC ELISA kit |
| Specificity | This kit recognizes Human PROC in samples. No significant cross-reactivity or interference between Human PROC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-106 | 99 |
| EDTA plasma (n=8) | 86-99 | 92 |
| Cell culture media (n=8) | 92-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 189.18 | 548.62 | 1879.79 | 202.95 | 578.76 | 1843.26 |
| Standard deviation | 12.54 | 30.67 | 78.20 | 10.70 | 31.20 | 101.19 |
| CV (%) | 6.63 | 5.59 | 4.16 | 5.27 | 5.39 | 5.49 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
