| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.47pmol/mL |
| Detection Limit: |
0.78~50pmol/mL |
| Short Description: |
This Pro-ADM Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
ADM |
| Gene ID: |
133 |
| Uniprot ID: |
ADML_HUMAN |
| Specificity: |
This kit recognizes Human Pro-ADM in samples. No significant cross-reactivity or interference between Human Pro-ADM and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. |
| Protein Name | Pro-Adrenomedullin Cleaved Into - AdrenomedullinAm - Proadrenomedullin N-20 Terminal PeptideProam N-Terminal 20 PeptidePampProam-N20 |
| Database Links | Reactome: R-HSA-418555Reactome: R-HSA-419812 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Pro-Adrenomedullin Cleaved Into - Adrenomedullin ELISA kitAm - Proadrenomedullin N-20 Terminal Peptide ELISA kitProam N-Terminal 20 Peptide ELISA kitPamp ELISA kitProam-N20 ELISA kitADM ELISA kitAM ELISA kit |
| Specificity | This kit recognizes Human Pro-ADM in samples. No significant cross-reactivity or interference between Human Pro-ADM and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-105 | 99 |
| EDTA plasma (n=8) | 95-107 | 101 |
| Cell culture media (n=8) | 94-109 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pmol/mL) | 2.43 | 7.94 | 19.47 | 2.37 | 7.20 | 18.19 |
| Standard deviation | 0.14 | 0.39 | 0.81 | 0.14 | 0.39 | 0.65 |
| CV (%) | 5.76 | 4.91 | 4.16 | 5.91 | 5.42 | 3.57 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
