| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.117ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This PRNP Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
PRNP |
| Gene ID: |
5621 |
| Uniprot ID: |
PRIO_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion. |
| Function | Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains. |
| Protein Name | Major Prion ProteinPrpAscrPrp27-30Prp33-35cCd Antigen Cd230 |
| Database Links | Reactome: R-HSA-419037Reactome: R-HSA-9609523 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorGolgi ApparatusTargeted To Lipid Rafts Via Association With The Heparan Sulfate Chains Of Gpc1ColocatesIn The Presence Of Cu(2+)To Vesicles In Para- And Perinuclear RegionsWhere Both Proteins Undergo InternalizationHeparin Displaces Prnp From Lipid Rafts And Promotes Endocytosis |
| Alternative ELISA Names | Major Prion Protein ELISA kitPrp ELISA kitAscr ELISA kitPrp27-30 ELISA kitPrp33-35c ELISA kitCd Antigen Cd230 ELISA kitPRNP ELISA kitALTPRP ELISA kitPRIP ELISA kitPRP ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
