| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.75ng/mL |
| Detection Limit: |
1.25~80ng/mL |
| Short Description: |
This PRG4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
PRG4 |
| Gene ID: |
10216 |
| Uniprot ID: |
PRG4_HUMAN |
| Specificity: |
This kit recognizes Human PRG4 in samples. No significant cross-reactivity or interference between Human PRG4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. O-glycosylated.contains glycosaminoglycan chondroitin sulfate and keratan sulfate. O-glycosylated with sialylated oligosaccharides which are predominantly represented by the monosialylated core type I structure, NeuNAcalpha2-3Galbeta1-3GalNAc, with smaller amounts of disialylated O-glycans. The disulfide bond between Cys-1146 and Cys-1403 is essential for protein cleavage. Proteolytically cleaved by cathepsin CTSG. |
| Function | Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages. |
| Protein Name | Proteoglycan 4LubricinMegakaryocyte-Stimulating FactorSuperficial Zone Proteoglycan Cleaved Into - Proteoglycan 4 C-Terminal Part |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Proteoglycan 4 ELISA kitLubricin ELISA kitMegakaryocyte-Stimulating Factor ELISA kitSuperficial Zone Proteoglycan Cleaved Into - Proteoglycan 4 C-Terminal Part ELISA kitPRG4 ELISA kitMSF ELISA kitSZP ELISA kit |
| Specificity | This kit recognizes Human PRG4 in samples. No significant cross-reactivity or interference between Human PRG4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-101 | 96 |
| EDTA plasma (n=8) | 94-107 | 99 |
| Cell culture media (n=8) | 94-110 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.43 | 10.47 | 30.78 | 4.53 | 10.91 | 27.96 |
| Standard deviation | 0.27 | 0.44 | 1.51 | 0.26 | 0.47 | 0.91 |
| CV (%) | 6.09 | 4.20 | 4.91 | 5.74 | 4.31 | 3.25 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
