| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human PRB2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PRB2 |
| Gene ID: |
653247 |
| Uniprot ID: |
PRB2_HUMAN |
| Specificity: |
This kit recognizes Human PRB2 in samples. No significant cross-reactivity or interference between Human PRB2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N- and O-glycosylated. In head and neck cancer patients, O-glycosylated with glucosylgalactosyl carbohydrate moiety. This modification would require prior hydroxylation on the lysine residue. Proteolytically cleaved at the tripeptide Xaa-Pro-Gln, where Xaa in the P(3) position is mostly lysine. The endoprotease may be of microbial origin. Pyroglutamate formation occurs on terminal Gln residues of cleaved peptides. Pyroglutamate formation found on at least Gln-398 and Gln-400. |
| Protein Name | Basic Salivary Proline-Rich Protein 2Salivary Proline-Rich ProteinCon1 Glycoprotein Cleaved Into - Basic Proline-Rich Peptide Ib-1 - Basic Proline-Rich Peptide P-EIb-9 - Basic Proline-Rich Peptide Ib-7 - Basic Proline-Rich Peptide Ib-8cBasic Peptide P-F - Basic Proline-Rich Peptide Ib-4 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Basic Salivary Proline-Rich Protein 2 CLIA kitSalivary Proline-Rich Protein CLIA kitCon1 Glycoprotein Cleaved Into - Basic Proline-Rich Peptide Ib-1 - Basic Proline-Rich Peptide P-E CLIA kitIb-9 - Basic Proline-Rich Peptide Ib-7 - Basic Proline-Rich Peptide Ib-8c CLIA kitBasic Peptide P-F - Basic Proline-Rich Peptide Ib-4 CLIA kitPRB2 CLIA kit |
| Specificity | This kit recognizes Human PRB2 in samples. No significant cross-reactivity or interference between Human PRB2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-116 | 107 |
| EDTA plasma(n=8) | 90-105 | 97 |
| Cell culture media(n=8) | 88-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 54.95 | 148.39 | 477.86 | 55.21 | 147.30 | 457.85 |
| Standard deviation | 6.12 | 15.51 | 30.30 | 5.15 | 15.83 | 44.92 |
| CV (%) | 11.14 | 10.45 | 6.34 | 9.33 | 10.75 | 9.81 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
