| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.117ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This PLCz1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
PLCZ1 |
| Gene ID: |
89869 |
| Uniprot ID: |
PLCZ1_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed specifically in testis and sperm. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis. |
| Post Translational Modifications | |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. |
| Protein Name | 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Zeta-1Phosphoinositide Phospholipase C-Zeta-1Phospholipase C-Zeta-1Plc-Zeta-1Testis-Development Protein Nyd-Sp27 |
| Database Links | Reactome: R-HSA-1855204 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionExhibits Alternative Cytoplasmic/Nuclear Localization During DevelopmentTranslocates From The Pronucleus Into Cytoplasm Upon Nuclear Envelope Breakdown For Mitosis And Localizes Again To The Pronucleus At Interphase Following Meiosis And Mitosis |
| Alternative ELISA Names | 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Zeta-1 ELISA kitPhosphoinositide Phospholipase C-Zeta-1 ELISA kitPhospholipase C-Zeta-1 ELISA kitPlc-Zeta-1 ELISA kitTestis-Development Protein Nyd-Sp27 ELISA kitPLCZ1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
