| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.068ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This PLCg1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
PLCG1 |
| Gene ID: |
5335 |
| Uniprot ID: |
PLCG1_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA. Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells. Ubiquitinated by CBLB in activated T-cells. |
| Function | Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. |
| Protein Name | 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-1Plc-148Phosphoinositide Phospholipase C-Gamma-1Phospholipase C-IiPlc-IiPhospholipase C-Gamma-1Plc-Gamma-1 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-1236382Reactome: R-HSA-1251932Reactome: R-HSA-1489509Reactome: R-HSA-167021Reactome: R-HSA-1855204Reactome: R-HSA-186763Reactome: R-HSA-201556Reactome: R-HSA-202433Reactome: R-HSA-2029485Reactome: R-HSA-210990Reactome: R-HSA-212718Reactome: R-HSA-2424491Reactome: R-HSA-2871796Reactome: R-HSA-2871809Reactome: R-HSA-418890Reactome: R-HSA-5218921Reactome: R-HSA-5637810Reactome: R-HSA-5654219Reactome: R-HSA-5654221Reactome: R-HSA-5654227Reactome: R-HSA-5654228Reactome: R-HSA-5655253Reactome: R-HSA-5655291Reactome: R-HSA-5655302Reactome: R-HSA-5655332Reactome: R-HSA-8853659Reactome: R-HSA-9026527Reactome: R-HSA-9027277Reactome: R-HSA-9034793Reactome: R-HSA-9664323Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9725370 |
| Cellular Localisation | Cell ProjectionLamellipodiumRuffleRapidly Redistributed To Ruffles And Lamellipodia Structures In Response To Epidermal Growth Factor (Egf) Treatment |
| Alternative ELISA Names | 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-1 ELISA kitPlc-148 ELISA kitPhosphoinositide Phospholipase C-Gamma-1 ELISA kitPhospholipase C-Ii ELISA kitPlc-Ii ELISA kitPhospholipase C-Gamma-1 ELISA kitPlc-Gamma-1 ELISA kitPLCG1 ELISA kitPLC1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
