| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human PLAUR kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PLAUR |
| Gene ID: |
5329 |
| Uniprot ID: |
UPAR_HUMAN |
| Specificity: |
This kit recognizes Human PLAUR/uPAR in samples. No significant cross-reactivity or interference between Human PLAUR/uPAR and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. |
| Function | Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. |
| Protein Name | Urokinase Plasminogen Activator Surface ReceptorU-ParUparMonocyte Activation Antigen Mo3Cd Antigen Cd87 |
| Database Links | Reactome: R-HSA-162791Reactome: R-HSA-6798695Reactome: R-HSA-75205 |
| Cellular Localisation | Cell MembraneCell ProjectionInvadopodium MembraneColocalized With Fap (Seprase) Preferentially At The Cell Surface Of Invadopodia Membrane In A Cytoskeleton-Integrin- And Vitronectin-Dependent MannerIsoform 1: Cell MembraneLipid-AnchorGpi-AnchorIsoform 2: Secreted |
| Alternative CLIA Names | Urokinase Plasminogen Activator Surface Receptor CLIA kitU-Par CLIA kitUpar CLIA kitMonocyte Activation Antigen Mo3 CLIA kitCd Antigen Cd87 CLIA kitPLAUR CLIA kitMO3 CLIA kitUPAR CLIA kit |
| Specificity | This kit recognizes Human PLAUR/uPAR in samples. No significant cross-reactivity or interference between Human PLAUR/uPAR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-98 | 93 |
| EDTA plasma(n=8) | 91-103 | 97 |
| Cell culture media(n=8) | 88-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 54.35 | 153.38 | 428.54 | 58.05 | 159.01 | 409.38 |
| Standard deviation | 6.10 | 17.50 | 45.51 | 5.57 | 12.24 | 30.58 |
| CV (%) | 11.22 | 11.41 | 10.62 | 9.60 | 7.70 | 7.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
