• Human PLA2G5 (Phospholipase A2, Group V) Sandwich ELISA Kit Ready-To-Use (STJE0017919)

Human PLA2G5 (Phospholipase A2, Group V) Sandwich ELISA Kit Ready-To-Use (STJE0017919)

SKU:
STJE0017919

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.105ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This PLA2G5 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: PLA2G5
Gene ID: 5322
Uniprot ID: PA2G5_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity Heart, placenta and less abundantly, in lung. Detected in the outer and inner plexiform layers of the retina (at protein level). Expressed in monocytes and macrophages.
Post Translational Modifications This enzyme lacks one of the seven disulfide bonds found in similar PLA2 proteins.
Function Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity), preferentially releasing fatty acyl groups with a low degree of unsaturation such as oleoyl (C18:1) and linoleoyl (C18:2) groups. Hydrolyzes low-density lipoprotein (LDL) phospholipids releasing unsaturated fatty acids that drive macrophage polarization toward an M2 phenotype. May act in an autocrine and paracrine manner. Contributes to lipid remodeling of cellular membranes at different subcellular locations and generation of lipid mediators involved in pathogen clearance. Cleaves sn-2 fatty acyl chains of cardiolipin, a major component of the inner membrane of mitochondria and bacterial membranes. Promotes phagocytosis of bacteria in macrophages through production of lysophosphatidylethanolamines. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Promotes phagocytosis and killing of ingested fungi likely through controlling phagosome-lysosome fusion and phagosome maturation. Plays a role in biosynthesis of cysteinyl leukotrienes (CysLTs) in myeloid cells. In eosinophils, triggers perinuclear arachidonate release and LTC4 synthesis in a PLA2G4A-independent way. In neutrophils, amplifies CysLTs biosynthesis initiated by PLA2G4A. Promotes immune complex clearance in macrophages via stimulating synthesis of CysLTs, which act through CYSLTR1 to trigger phagocytosis. May regulate antigen processing in antigen-presenting cells. In pulmonary macrophages regulates IL33 production required for activation of group 2 innate lymphoid cells. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines.
Protein Name Phospholipase A2 Group V
Pla2-10
Phosphatidylcholine 2-Acylhydrolase 5
Database Links Reactome: R-HSA-1482788
Reactome: R-HSA-1482801
Reactome: R-HSA-1482839
Reactome: R-HSA-1482922
Reactome: R-HSA-1482925
Reactome: R-HSA-1483166
Cellular Localisation Secreted
Cell Membrane
Cytoplasmic Vesicle
Phagosome
Recycling Endosome
Golgi Apparatus
Cis-Golgi Network
Trans-Golgi Network
Alternative ELISA Names Phospholipase A2 Group V ELISA kit
Pla2-10 ELISA kit
Phosphatidylcholine 2-Acylhydrolase 5 ELISA kit
PLA2G5 ELISA kit
output

Information sourced from Uniprot.org

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