| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38IU/mL |
| Detection Limit: |
15.63~1000IU/mL |
| Short Description: |
This PKM Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
PKM |
| Gene ID: |
5315 |
| Uniprot ID: |
KPYM_HUMAN |
| Specificity: |
This kit recognizes Human PKM2 in samples. No significant cross-reactivity or interference between Human PKM2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. Isoform M2: Acetylated at Lys-433 by EP300, leading to impair phosphoenolpyruvate substrate-binding and promote its homodimerization and subsequent translocation to the nucleus. Deacetylation at Lys-433 by SIRT6 promotes its nuclear export into the cytoplasm, leading to suppress its nuclear localization and oncogenic function. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. |
| Function | Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Isoform M2: Isoform specifically expressed during embryogenesis that has low pyruvate kinase activity by itself and requires allosteric activation by D-fructose 1,6-bisphosphate (FBP) for pyruvate kinase activity. In addition to its pyruvate kinase activity in the cytoplasm, also acts as a regulator of transcription in the nucleus by acting as a protein kinase. Translocates into the nucleus in response to various signals, such as EGF receptor activation, and homodimerizes, leading to its conversion into a protein threonine- and tyrosine-protein kinase. Catalyzes phosphorylation of STAT3 at 'Tyr-705' and histone H3 at 'Thr-11' (H3T11ph), leading to activate transcription. Its ability to activate transcription plays a role in cancer cells by promoting cell proliferation and promote tumorigenesis. Promotes the expression of the immune checkpoint protein CD274 in BMAL1-deficient macrophages. May also act as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs. Plays a role in caspase independent cell death of tumor cells. Isoform M1: Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth. In contrast to isoform M2, has high pyruvate kinase activity by itself and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP) for activity. |
| Protein Name | Pyruvate Kinase PkmCytosolic Thyroid Hormone-Binding ProteinCthbpOpa-Interacting Protein 3Oip-3Pyruvate Kinase 2/3Pyruvate Kinase Muscle IsozymeThreonine-Protein Kinase Pkm2Thyroid Hormone-Binding Protein 1Thbp1Tumor M2-PkTyrosine-Protein Kinase Pkm2P58 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-70171 |
| Cellular Localisation | Isoform M2: CytoplasmNucleusTranslocates To The Nucleus In Response To Various SignalsSuch As Egf Receptor Activation Or Apoptotic StimuliNuclear Translocation Is Promoted By Acetylation By Ep300Deacetylation By Sirt6 Promotes Its Nuclear Export In A Process Dependent Of Xpo4Thereby Suppressing Its Ability To Activate Transcription And Promote TumorigenesisIsoform M1: Cytoplasm |
| Alternative ELISA Names | Pyruvate Kinase Pkm ELISA kitCytosolic Thyroid Hormone-Binding Protein ELISA kitCthbp ELISA kitOpa-Interacting Protein 3 ELISA kitOip-3 ELISA kitPyruvate Kinase 2/3 ELISA kitPyruvate Kinase Muscle Isozyme ELISA kitThreonine-Protein Kinase Pkm2 ELISA kitThyroid Hormone-Binding Protein 1 ELISA kitThbp1 ELISA kitTumor M2-Pk ELISA kitTyrosine-Protein Kinase Pkm2 ELISA kitP58 ELISA kitPKM ELISA kitOIP3 ELISA kitPK2 ELISA kitPK3 ELISA kitPKM2 ELISA kit |
| Specificity | This kit recognizes Human PKM2 in samples. No significant cross-reactivity or interference between Human PKM2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 95 |
| EDTA plasma (n=8) | 98-110 | 105 |
| Cell culture media (n=8) | 90-99 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (IU/mL) | 47.47 | 116.17 | 471.44 | 47.83 | 121.50 | 409.39 |
| Standard deviation | 2.69 | 5.66 | 22.06 | 2.50 | 5.67 | 17.19 |
| CV (%) | 5.67 | 4.87 | 4.68 | 5.23 | 4.67 | 4.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
