• Human PKBg (Protein Kinase B Gamma) Sandwich ELISA Kit (STJE0011494)

Human PKBg (Protein Kinase B Gamma) Sandwich ELISA Kit (STJE0011494)

SKU:
STJE0011494

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.56ng/mL
Detection Limit: 1.56-100ng/mL
Short Description: This PKBg Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: AKT3
Gene ID: 10000
Uniprot ID: AKT3_HUMAN
Sample Type: tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Post Translational Modifications Phosphorylation on Thr-305 and Ser-472 is required for full activity. Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1.
Function AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Protein Name Rac-Gamma Serine/Threonine-Protein Kinase
Protein Kinase Akt-3
Protein Kinase B Gamma
Pkb Gamma
Rac-Pk-Gamma
Stk-2
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1257604
Reactome: R-HSA-1358803
Reactome: R-HSA-198323
Reactome: R-HSA-198693
Reactome: R-HSA-199418
Reactome: R-HSA-211163
Reactome: R-HSA-389357
Reactome: R-HSA-389513
Reactome: R-HSA-392451
Reactome: R-HSA-5218920
Reactome: R-HSA-5628897
Reactome: R-HSA-5674400
Reactome: R-HSA-6804757
Reactome: R-HSA-6804758
Reactome: R-HSA-6804759
Reactome: R-HSA-69202
Reactome: R-HSA-69656
Reactome: R-HSA-8876198
Reactome: R-HSA-8941332
Reactome: R-HSA-8948751
Reactome: R-HSA-9607240
Reactome: R-HSA-9614399
Reactome: R-HSA-9634638
Reactome: R-HSA-9755511
Reactome: R-HSA-9755779
Cellular Localisation Nucleus
Cytoplasm
Membrane
Peripheral Membrane Protein
Membrane-Associated After Cell Stimulation Leading To Its Translocation
Alternative ELISA Names Rac-Gamma Serine/Threonine-Protein Kinase ELISA kit
Protein Kinase Akt-3 ELISA kit
Protein Kinase B Gamma ELISA kit
Pkb Gamma ELISA kit
Rac-Pk-Gamma ELISA kit
Stk-2 ELISA kit
AKT3 ELISA kit
PKBG ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance