• Human PIK3Cb (Phosphoinositide-3-Kinase Catalytic Beta Polypeptide) Sandwich ELISA Kit (STJE0011473)

Human PIK3Cb (Phosphoinositide-3-Kinase Catalytic Beta Polypeptide) Sandwich ELISA Kit (STJE0011473)

SKU:
STJE0011473

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.059ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This PIK3Cb Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: PIK3CB
Gene ID: 5291
Uniprot ID: PK3CB_HUMAN
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Expressed ubiquitously.
Post Translational Modifications Autophosphorylation at Ser-1070 negatively regulates the phosphatidylinositol-4,5-bisphosphate 3-kinase activity.
Function Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. Has also a protein kinase activity showing autophosphorylation.
Protein Name Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Beta Isoform
Pi3-Kinase Subunit Beta
Pi3k-Beta
Pi3kbeta
Ptdins-3-Kinase Subunit Beta
Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Beta
Ptdins-3-Kinase Subunit P110-Beta
P110beta
Serine/Threonine Protein Kinase Pik3cb
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-112399
Reactome: R-HSA-114604
Reactome: R-HSA-1257604
Reactome: R-HSA-1660499
Reactome: R-HSA-186763
Reactome: R-HSA-198203
Reactome: R-HSA-201556
Reactome: R-HSA-202424
Reactome: R-HSA-2029485
Reactome: R-HSA-210993
Reactome: R-HSA-2219530
Reactome: R-HSA-2424491
Reactome: R-HSA-2730905
Reactome: R-HSA-373753
Reactome: R-HSA-4420097
Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-8853659
Reactome: R-HSA-9027276
Reactome: R-HSA-912526
Reactome: R-HSA-912631
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-9680350
Cellular Localisation Cytoplasm
Nucleus
Interaction With Pik3r2 Is Required For Nuclear Localization And Export
Alternative ELISA Names Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Beta Isoform ELISA kit
Pi3-Kinase Subunit Beta ELISA kit
Pi3k-Beta ELISA kit
Pi3kbeta ELISA kit
Ptdins-3-Kinase Subunit Beta ELISA kit
Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Beta ELISA kit
Ptdins-3-Kinase Subunit P110-Beta ELISA kit
P110beta ELISA kit
Serine/Threonine Protein Kinase Pik3cb ELISA kit
PIK3CB ELISA kit
PIK3C1 ELISA kit
output

Information sourced from Uniprot.org

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