• Human PIGR (Polymeric immunoglobulin receptor) Sandwich ELISA (STJE0002815)
  • Human PIGR (Polymeric immunoglobulin receptor) Sandwich ELISA (STJE0002815)

Human PIGR (Polymeric immunoglobulin receptor) Sandwich ELISA (STJE0002815)

SKU:
STJE0002815

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This PIGR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PIGR
Gene ID: 5284
Uniprot ID: PIGR_HUMAN
Specificity: This kit recognizes Human PIGR/SC in samples. No significant cross-reactivity or interference between Human PIGR/SC and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Polymeric immunoglobulin receptor: Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. Secretory component: Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens. On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells.
Protein Name Polymeric Immunoglobulin Receptor
Pigr
Poly-Ig Receptor
Hepatocellular Carcinoma-Associated Protein Tb6 Cleaved Into - Secretory Component
Database Links Reactome: R-HSA-6798695
Cellular Localisation Polymeric Immunoglobulin Receptor: Cell Membrane
Single-Pass Type I Membrane Protein
Secretory Component: Secreted
Alternative ELISA Names Polymeric Immunoglobulin Receptor ELISA kit
Pigr ELISA kit
Poly-Ig Receptor ELISA kit
Hepatocellular Carcinoma-Associated Protein Tb6 Cleaved Into - Secretory Component ELISA kit
PIGR ELISA kit
Specificity This kit recognizes Human PIGR/SC in samples. No significant cross-reactivity or interference between Human PIGR/SC and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-101 92
EDTA plasma (n=8) 89-100 95
Cell culture media (n=8) 91-109 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 100.27 252.56 732.10 102.39 271.44 758.06
Standard deviation 6.00 10.58 37.56 5.53 12.46 28.12
CV (%) 5.98 4.19 5.13 5.40 4.59 3.71
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance