| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This PIBF1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
PIBF1 |
| Gene ID: |
10464 |
| Uniprot ID: |
PIBF1_HUMAN |
| Specificity: |
This kit recognizes Human PIBF in samples. No significant cross-reactivity or interference between Human PIBF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Plays a role in ciliogenesis. Isoform 1: Pericentriolar protein required to maintain mitotic spindle pole integrity. Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation. Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2. Isoform 4: The secreted form is a mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling decidual naturakl killer cells (NK) activity exerts an anti-abortive effect. Increases the production of Th2-type cytokines by signaling via the JAK/STAT pathway. Activates STAT6 and inhibits STAT4 phosphorylation. Signaling via a not identified receptor seems to implicate IL4R and a GPI-anchored protein. |
| Protein Name | Progesterone-Induced-Blocking Factor 1PibfCentrosomal Protein Of 90 KdaCep90 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSecretedIn Progesterone-Treated Astrocytoma Cells A 57 Kda Protein And Isoform 1 (90 Kda) Have Been DescribedBoth Being Located In The Intracellular Medium And SecretedRespective Predominant Forms Are Isoform 1 In The Intracellular And The 57 Kda Protein In The Extracellular MediumIsoform 1: NucleusCentriolar SatelliteLocalizes To Centriolar Satellites Throughout The Cell CycleIsoform 4: SecretedSecreted By Progesterone-Treated Lymphocytes |
| Alternative ELISA Names | Progesterone-Induced-Blocking Factor 1 ELISA kitPibf ELISA kitCentrosomal Protein Of 90 Kda ELISA kitCep90 ELISA kitPIBF1 ELISA kitC13orf24 ELISA kitPIBF ELISA kit |
| Specificity | This kit recognizes Human PIBF in samples. No significant cross-reactivity or interference between Human PIBF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-107 | 100 |
| EDTA plasma (n=8) | 93-106 | 98 |
| Cell culture media (n=8) | 95-106 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.09 | 2.84 | 8.62 | 1.10 | 2.89 | 8.03 |
| Standard deviation | 0.06 | 0.14 | 0.46 | 0.08 | 0.14 | 0.31 |
| CV (%) | 5.50 | 4.93 | 5.34 | 7.27 | 4.84 | 3.86 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
