| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This PGLYRP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
PGLYRP1 |
| Gene ID: |
8993 |
| Uniprot ID: |
PGRP1_HUMAN |
| Specificity: |
This kit recognizes Human PGRP-S in samples. No significant cross-reactivity or interference between Human PGRP-S and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. N-glycosylation is required for bactericidal activity. |
| Function | Innate immunity protein that plays several important functions in antimicrobial and antitumor defense systems. Acts as a pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria and thus provides bactericidal activity. Forms an equimolar complex with heat shock protein HSPA1A and induces programmed cell death through apoptosis and necroptosis in tumor cell lines by activating the TNFR1 receptor on the target cell membrane. In addition, acts in complex with the Ca(2+)-binding protein S100A4 as a chemoattractant able to induce lymphocyte movement. Mechanistically, this complex acts as a ligand of the chemotactic receptors CCR5 and CXCR3 which are present on the cells of the immune system. Promotes also the activation of lymphocytes that become able to kill virus-infected cells as well as tumor cells by modulating the spectrum of their target-cell specificity. Induction of cytotoxicity on monocyte surface requires interaction with TREM1 receptor. |
| Protein Name | Peptidoglycan Recognition Protein 1Peptidoglycan Recognition Protein ShortPgrp-S |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-6803157 |
| Cellular Localisation | SecretedCytoplasmic Granule |
| Alternative ELISA Names | Peptidoglycan Recognition Protein 1 ELISA kitPeptidoglycan Recognition Protein Short ELISA kitPgrp-S ELISA kitPGLYRP1 ELISA kitPGLYRP ELISA kitPGRP ELISA kitTNFSF3L ELISA kitSBBI68 ELISA kitUNQ639 ELISA kitPRO1269 ELISA kit |
| Specificity | This kit recognizes Human PGRP-S in samples. No significant cross-reactivity or interference between Human PGRP-S and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-110 | 100 |
| EDTA plasma (n=8) | 83-97 | 90 |
| Cell culture media (n=8) | 86-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.93 | 2.75 | 8.01 | 1.01 | 2.90 | 8.66 |
| Standard deviation | 0.05 | 0.16 | 0.27 | 0.05 | 0.16 | 0.47 |
| CV (%) | 5.38 | 5.82 | 3.37 | 4.95 | 5.52 | 5.43 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
