| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This human PF4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PF4 |
| Gene ID: |
5196 |
| Uniprot ID: |
PLF4_HUMAN |
| Specificity: |
This kit recognizes Human PF4 in samples. No significant cross-reactivity or interference between Human PF4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. |
| Protein Name | Platelet Factor 4Pf-4C-X-C Motif Chemokine 4IroplactOncostatin-A Cleaved Into - Platelet Factor 4 - Short FormEndothelial Cell Growth Inhibitor |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-140875Reactome: R-HSA-202733Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-8936459 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Platelet Factor 4 CLIA kitPf-4 CLIA kitC-X-C Motif Chemokine 4 CLIA kitIroplact CLIA kitOncostatin-A Cleaved Into - Platelet Factor 4 - Short Form CLIA kitEndothelial Cell Growth Inhibitor CLIA kitPF4 CLIA kitCXCL4 CLIA kitSCYB4 CLIA kit |
| Specificity | This kit recognizes Human PF4 in samples. No significant cross-reactivity or interference between Human PF4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-116 | 106 |
| EDTA plasma(n=8) | 98-116 | 106 |
| Cell culture media(n=8) | 88-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.93 | 2.17 | 8.85 | 0.90 | 2.17 | 8.10 |
| Standard deviation | 0.09 | 0.18 | 0.78 | 0.09 | 0.17 | 0.69 |
| CV (%) | 9.68 | 8.29 | 8.81 | 10.00 | 7.83 | 8.52 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
