Host: | HEK293 cells |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Recombinant-Human PDGFR-beta/CD140b-C-His & Avi protein was developed from hek293 cells and has a target region of C-His & Avi. For use in research applications. |
Conjugation: | Biotin |
Formulation: | Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | PDGFRB |
Gene ID: | 5159 |
Uniprot ID: | PGFRB_HUMAN |
Immunogen Region: | Leu33-Phe530 |
Immunogen: | Biotinylated Recombinant Human PDGF R beta Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Leu33-Phe530) of Human PDGF R beta fused with His tag and Avi tag at the C-terminal. |
Function | Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor. |
Protein Name | Platelet-Derived Growth Factor Receptor Beta Pdgf-R-Beta Pdgfr-Beta Beta Platelet-Derived Growth Factor Receptor Beta-Type Platelet-Derived Growth Factor Receptor Cd140 Antigen-Like Family Member B Platelet-Derived Growth Factor Receptor 1 Pdgfr-1 Cd Antigen Cd140b |
Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-186763 Reactome: R-HSA-186797 Reactome: R-HSA-2219530 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cytoplasmic Vesicle Lysosome Lumen After Ligand Binding The Autophosphorylated Receptor Is Ubiquitinated And Internalized Leading To Its Degradation |
Alternative Protein Names | Platelet-Derived Growth Factor Receptor Beta protein Pdgf-R-Beta protein Pdgfr-Beta protein Beta Platelet-Derived Growth Factor Receptor protein Beta-Type Platelet-Derived Growth Factor Receptor protein Cd140 Antigen-Like Family Member B protein Platelet-Derived Growth Factor Receptor 1 protein Pdgfr-1 protein Cd Antigen Cd140b protein PDGFRB protein PDGFR protein PDGFR1 protein |
Information sourced from Uniprot.org