| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.27ng/mL |
| Detection Limit: |
0.781-50ng/mL |
| Short Description: |
This PARN Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
PARN |
| Gene ID: |
5073 |
| Uniprot ID: |
PARN_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation by MAPKAPK2, preventing GADD45A mRNA degradation after genotoxic stress. |
| Function | 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Also able to recognize and trim poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to microRNAs degradation or snoRNA increased stability. |
| Protein Name | Poly(A-Specific Ribonuclease ParnDeadenylating NucleaseDeadenylation NucleasePolyadenylate-Specific Ribonuclease |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-429947Reactome: R-HSA-450604 |
| Cellular Localisation | NucleusCytoplasmNucleolusSome Nuclear Fraction Is Nucleolar |
| Alternative ELISA Names | Poly(A-Specific Ribonuclease Parn ELISA kitDeadenylating Nuclease ELISA kitDeadenylation Nuclease ELISA kitPolyadenylate-Specific Ribonuclease ELISA kitPARN ELISA kitDAN ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
