| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
13.5pg/mL |
| Detection Limit: |
31.2-2000pg/mL |
| Short Description: |
This PAM Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
PAM |
| Gene ID: |
5066 |
| Uniprot ID: |
AMD_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate. |
| Protein Name | Peptidyl-Glycine Alpha-Amidating MonooxygenasePam Includes - Peptidylglycine Alpha-Hydroxylating MonooxygenasePhm - Peptidyl-Alpha-Hydroxyglycine Alpha-Amidating LyasePeptidylamidoglycolate LyasePal |
| Database Links | |
| Cellular Localisation | Cytoplasmic VesicleSecretory Vesicle MembraneSingle-Pass Membrane ProteinSecretory GranulesIsoform 1: MembraneSingle-Pass Type I Membrane ProteinIsoform 2: MembraneIsoform 3: SecretedSecreted From Secretory GranulesIsoform 4: Secreted |
| Alternative ELISA Names | Peptidyl-Glycine Alpha-Amidating Monooxygenase ELISA kitPam Includes - Peptidylglycine Alpha-Hydroxylating Monooxygenase ELISA kitPhm - Peptidyl-Alpha-Hydroxyglycine Alpha-Amidating Lyase ELISA kitPeptidylamidoglycolate Lyase ELISA kitPal ELISA kitPAM ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
