• Human PAH (Phenylalanine-4-hydroxylase) Sandwich ELISA (STJE0003005)
  • Human PAH (Phenylalanine-4-hydroxylase) Sandwich ELISA (STJE0003005)

Human PAH (Phenylalanine-4-hydroxylase) Sandwich ELISA (STJE0003005)

SKU:
STJE0003005

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This PAH Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PAH
Gene ID: 5053
Uniprot ID: PH4H_HUMAN
Specificity: This kit recognizes Human PAH in samples. No significant cross-reactivity or interference between Human PAH and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Protein Name Phenylalanine-4-Hydroxylase
Pah
Phe-4-Monooxygenase
Database Links Reactome: R-HSA-2160456
Reactome: R-HSA-8964208
Alternative ELISA Names Phenylalanine-4-Hydroxylase ELISA kit
Pah ELISA kit
Phe-4-Monooxygenase ELISA kit
PAH ELISA kit
Specificity This kit recognizes Human PAH in samples. No significant cross-reactivity or interference between Human PAH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-100 94
EDTA plasma (n=8) 93-108 99
Cell culture media (n=8) 92-104 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 252.26 581.22 1975.95 237.28 610.23 1869.07
Standard deviation 12.89 31.85 100.18 16.49 29.66 71.40
CV (%) 5.11 5.48 5.07 6.95 4.86 3.82
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance