| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.47ng/mL |
| Detection Limit: |
0.78~50ng/mL |
| Short Description: |
This human ORM1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ORM1 |
| Gene ID: |
5004 |
| Uniprot ID: |
A1AG1_HUMAN |
| Specificity: |
This kit recognizes Human AGP1 in samples. No significant cross-reactivity or interference between Human AGP1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| | rn | V1 | V2 |
| Tissue Specificity | Expressed by the liver and secreted in plasma. | Expressed by the liver and secreted in plasma. |
| Post Translational Modifications | N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor). | N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor). |
| Function | Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. | Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. |
| Protein Name | Alpha-1-Acid Glycoprotein 1Agp 1Orosomucoid-1Omd 1 | Alpha-1-Acid Glycoprotein 1Agp 1Orosomucoid-1Omd 1 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695 | Reactome: R-HSA-114608Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted | Secreted |
| Alternative CLIA Names | Alpha-1-Acid Glycoprotein 1 CLIA kitAgp 1 CLIA kitOrosomucoid-1 CLIA kitOmd 1 CLIA kitORM1 CLIA kitAGP1 CLIA kit | Alpha-1-Acid Glycoprotein 1 CLIA kitAgp 1 CLIA kitOrosomucoid-1 CLIA kitOmd 1 CLIA kitORM1 CLIA kitAGP1 CLIA kit |
| Specificity | This kit recognizes Human AGP1 in samples. No significant cross-reactivity or interference between Human AGP1 and analogues was observed. | This kit recognizes Human AGP1 in samples. No significant cross-reactivity or interference between Human AGP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-111 | 106 |
| EDTA plasma(n=8) | 96-110 | 102 |
| Cell culture media(n=8) | 100-113 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.51 | 4.56 | 18.74 | 2.62 | 4.79 | 18.41 |
| Standard deviation | 0.28 | 0.44 | 1.65 | 0.25 | 0.54 | 1.97 |
| CV (%) | 11.16 | 9.65 | 8.80 | 9.54 | 11.27 | 10.70 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
