Recombinant Human Orexin-A/Hypocretin/HCRT Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human Orexin-A/Hypocretin/HCRT protein (Recombinant) (C-His) (STJP002991)

SKU:
STJP002991
£106.50 - £359.50
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% Trehalose is added as protectant before lyophilisation.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: < 0.1 EU/Mu g of the protein by LAL method
Gene Symbol: HCRT
Gene ID: 3060
Uniprot ID: OREX_HUMAN
Immunogen Region: Gln34-Leu66
Immunogen: Recombinant Human Orexin-A/Hypocretin/HCRT Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln34-Leu66) of Human Orexin A/Hypocretin-1 (Accession #NP_001515.1) fused with a hFc tag at the N-termin
Immunogen Sequence: SREPLRPWCHPINAILAVEK EGCPVCITVNTTICAGYCPT MMRVLQAVLPPLPQVVCTYR DVRFESIRLPGCPRGVDPVV SFPVALSCRCGPCRRSTSDC GGPKDHPLTCDHPQLSGLLF L
Tissue Specificity Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications Specific enzymatic cleavages at paired basic residues yield the different active peptides.
Function Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A: Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. Orexin-B: Binds to orexin receptor HCRTR2/OX2R only. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner.
Protein Name Hypocretin Neuropeptide Precursor
Hypocretin
Hcrt
Orexin Precursor
Prepro-Orexin
Preprohypocretin Cleaved Into - Orexin-A
Hypocretin-1
Hcrt1 - Orexin-B
Hypocretin-2
Hcrt2
Database Links Reactome: R-HSA-389397
Reactome: R-HSA-416476
Cellular Localisation Rough Endoplasmic Reticulum
Cytoplasmic Vesicle
Synapse
Associated With Perikaryal Rough Endoplasmic Reticulum As Well As Cytoplasmic Large Granular Vesicles At Synapses
Alternative Protein Names Hypocretin Neuropeptide Precursor protein
Hypocretin protein
Hcrt protein
Orexin Precursor protein
Prepro-Orexin protein
Preprohypocretin Cleaved Into - Orexin-A protein
Hypocretin-1 protein
Hcrt1 - Orexin-B protein
Hypocretin-2 protein
Hcrt2 protein
HCRT protein
OX protein
PPORX protein
PPOX protein

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJE0002658 Human HCRT (Orexin A) Competitive ELISA Kit (STJE0002658) Human ELISA
STJP001209 Human LRRC4 protein (Recombinant) (C-His) (STJP001209) HEK293 cells
STJP001979 Human CD320 protein (Recombinant) (C-His) (STJP001979) HEK293 cells
STJP002429 Human Lactotransferrin protein (Recombinant) (C-His) (STJP002429) HEK293 cells
STJP003087 Human IgM protein (Recombinant) (C-His) (STJP003087) HEK293 cells

Related Products

!