• Human OGN (Mimecan) Sandwich ELISA (STJE0003499)

Human OGN (Mimecan) Sandwich ELISA (STJE0003499)

SKU:
STJE0003499

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description: This OGN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: OGN
Gene ID: 4969
Uniprot ID: MIME_HUMAN
Specificity: This kit recognizes Human OGN in samples. No significant cross-reactivity or interference between Human OGN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O-glycosylated with a core 1 or possibly core 8 glycan. Contains keratan sulfate.
Function Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
Protein Name Mimecan
Osteoglycin
Osteoinductive Factor
Oif
Database Links Reactome: R-HSA-2022854
Reactome: R-HSA-2022857
Reactome: R-HSA-3656225
Reactome: R-HSA-3656243
Reactome: R-HSA-3656244
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Mimecan ELISA kit
Osteoglycin ELISA kit
Osteoinductive Factor ELISA kit
Oif ELISA kit
OGN ELISA kit
OIF ELISA kit
SLRR3A ELISA kit
Specificity This kit recognizes Human OGN in samples. No significant cross-reactivity or interference between Human OGN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 95
EDTA plasma (n=8) 89-104 96
Cell culture media (n=8) 96-111 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.16 3.48 14.57 2.30 3.50 13.57
Standard deviation 0.13 0.14 0.53 0.14 0.15 0.41
CV (%) 6.02 4.02 3.64 6.09 4.29 3.02
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance