| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human NRP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
NRP1 |
| Gene ID: |
8829 |
| Uniprot ID: |
NRP1_HUMAN |
| Specificity: |
This kit recognizes Human NRP1 in samples. No significant cross-reactivity or interference between Human NRP1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 1: The expression of isoforms 1 and 2 does not seem to overlap. Expressed in olfactory epithelium (at protein level). Expressed in fibroblasts (at protein level). Expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta.moderately in lung, liver, skeletal muscle, kidney and pancreas.and low in adult brain. Expressed in the central nervous system, including olfactory related regions such as the olfactory tubercles and paraolfactory gyri. Isoform 2: The expression of isoforms 1 and 2 does not seem to overlap. Found in liver hepatocytes, kidney distal and proximal tubules. |
| Function | Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR. (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection. Isoform 2: Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. |
| Protein Name | Neuropilin-1Vascular Endothelial Cell Growth Factor 165 ReceptorCd Antigen Cd304 |
| Database Links | Reactome: R-HSA-194306Reactome: R-HSA-376176Reactome: R-HSA-399954Reactome: R-HSA-399955Reactome: R-HSA-399956Reactome: R-HSA-445144Reactome: R-HSA-447041Reactome: R-HSA-9694614 |
| Cellular Localisation | Isoform 2: SecretedMitochondrion MembraneSingle-Pass Type I Membrane ProteinCell MembraneCytoplasm |
| Alternative CLIA Names | Neuropilin-1 CLIA kitVascular Endothelial Cell Growth Factor 165 Receptor CLIA kitCd Antigen Cd304 CLIA kitNRP1 CLIA kitNRP CLIA kitVEGF165R CLIA kit |
| Specificity | This kit recognizes Human NRP1 in samples. No significant cross-reactivity or interference between Human NRP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 103 |
| EDTA plasma(n=8) | 101-117 | 107 |
| Cell culture media(n=8) | 98-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.58 | 146.97 | 353.82 | 56.30 | 134.77 | 355.49 |
| Standard deviation | 5.17 | 14.46 | 39.52 | 5.64 | 15.85 | 39.64 |
| CV (%) | 10.02 | 9.84 | 11.17 | 10.02 | 11.76 | 11.15 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
