| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human NR1I2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
NR1I2 |
| Gene ID: |
8856 |
| Uniprot ID: |
NR1I2_HUMAN |
| Specificity: |
This kit recognizes Human PXR in samples. No significant cross-reactivity or interference between Human PXR and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in liver, colon and small intestine. |
| Function | Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. |
| Protein Name | Nuclear Receptor Subfamily 1 Group I Member 2Orphan Nuclear Receptor Par1Orphan Nuclear Receptor PxrPregnane X ReceptorSteroid And Xenobiotic ReceptorSxr |
| Database Links | Reactome: R-HSA-383280Reactome: R-HSA-4090294 O75469-1 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Nuclear Receptor Subfamily 1 Group I Member 2 CLIA kitOrphan Nuclear Receptor Par1 CLIA kitOrphan Nuclear Receptor Pxr CLIA kitPregnane X Receptor CLIA kitSteroid And Xenobiotic Receptor CLIA kitSxr CLIA kitNR1I2 CLIA kitPXR CLIA kit |
| Specificity | This kit recognizes Human PXR in samples. No significant cross-reactivity or interference between Human PXR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-106 | 98 |
| EDTA plasma(n=8) | 95-111 | 101 |
| Cell culture media(n=8) | 92-104 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 104.20 | 206.55 | 908.49 | 111.55 | 203.46 | 981.23 |
| Standard deviation | 9.47 | 22.22 | 63.59 | 11.67 | 14.38 | 110.29 |
| CV (%) | 9.09 | 10.76 | 7.00 | 10.46 | 7.07 | 11.24 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
