| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.124ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This NQO1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
NQO1 |
| Gene ID: |
1728 |
| Uniprot ID: |
NQO1_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species. Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion. Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential. Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome. |
| Protein Name | Nad(Ph Dehydrogenase Quinone 1AzoreductaseDt-DiaphoraseDtdMenadione ReductaseNad(Ph -Quinone Oxidoreductase 1Phylloquinone ReductaseQuinone Reductase 1Qr1 |
| Database Links | Reactome: R-HSA-350562Reactome: R-HSA-9818027 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative ELISA Names | Nad(Ph Dehydrogenase Quinone 1 ELISA kitAzoreductase ELISA kitDt-Diaphorase ELISA kitDtd ELISA kitMenadione Reductase ELISA kitNad(Ph -Quinone Oxidoreductase 1 ELISA kitPhylloquinone Reductase ELISA kitQuinone Reductase 1 ELISA kitQr1 ELISA kitNQO1 ELISA kitDIA4 ELISA kitNMOR1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
