• Human NPYR2 (Neuropeptide Y Receptor Y2) Sandwich ELISA (STJE0002505)

Human NPYR2 (Neuropeptide Y Receptor Y2) Sandwich ELISA (STJE0002505)

SKU:
STJE0002505

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This NPYR2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: NPY2R
Gene ID: 4887
Uniprot ID: NPY2R_HUMAN
Specificity: This kit recognizes Human NPYR2 in samples. No significant cross-reactivity or interference between Human NPYR2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > Ile-31, Gln-34 PP > Leu-31, Pro-34 NPY > PP, Pro-34 PYY and NPY free acid.
Protein Name Neuropeptide Y Receptor Type 2
Npy2-R
Npy-Y2 Receptor
Y2 Receptor
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative ELISA Names Neuropeptide Y Receptor Type 2 ELISA kit
Npy2-R ELISA kit
Npy-Y2 Receptor ELISA kit
Y2 Receptor ELISA kit
NPY2R ELISA kit
Specificity This kit recognizes Human NPYR2 in samples. No significant cross-reactivity or interference between Human NPYR2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 98
EDTA plasma (n=8) 92-105 100
Cell culture media (n=8) 87-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.99 2.73 9.15 1.05 2.78 9.62
Standard deviation 0.05 0.12 0.48 0.06 0.16 0.50
CV (%) 5.05 4.40 5.25 5.71 5.76 5.20
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance