| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.128ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This NPM Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
NPM1 |
| Gene ID: |
4869 |
| Uniprot ID: |
NPM_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Acetylated at C-terminal lysine residues, thereby increasing affinity to histones. ADP-ribosylated. Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels. Sumoylated by ARF. Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36. |
| Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1. |
| Protein Name | NucleophosminNpmNucleolar Phosphoprotein B23Nucleolar Protein No38Numatrin |
| Database Links | Reactome: R-HSA-180746Reactome: R-HSA-3899300Reactome: R-HSA-606279Reactome: R-HSA-6804115Reactome: R-HSA-8869496Reactome: R-HSA-9692914Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9725371 |
| Cellular Localisation | NucleusNucleolusNucleoplasmCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeGenerally NucleolarBut Is Translocated To The Nucleoplasm In Case Of Serum Starvation Or Treatment With Anticancer DrugsHas Been Found In The Cytoplasm In Patients With Primary Acute Myelogenous Leukemia (Aml)But Not With Secondary AmlCan Shuttle Between Cytoplasm And NucleusCo- Localizes With The Methylated Form Of Rps10 In The Granular Component (Gc) Region Of The NucleolusColocalized With Nucleolin And Apex1 In NucleoliIsoform 1 Of Nek2 Is Required For Its Localization To The Centrosome During Mitosis |
| Alternative ELISA Names | Nucleophosmin ELISA kitNpm ELISA kitNucleolar Phosphoprotein B23 ELISA kitNucleolar Protein No38 ELISA kitNumatrin ELISA kitNPM1 ELISA kitNPM ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
