| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This NOX5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
NOX5 |
| Gene ID: |
79400 |
| Uniprot ID: |
NOX5_HUMAN |
| Specificity: |
This kit recognizes Human NOX5 in samples. No significant cross-reactivity or interference between Human NOX5 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Isoform v2: Phosphorylation at Ser-475 by CaMK2 and at Ser-490, Thr-494 and Ser-498 by PKC/PRKCA positively regulates its catalytic activity. Isoform v2: S-nitrosylation in response to nitric oxide inhibits its catalytic activity. |
| Function | Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. May play a role in cell growth and apoptosis. Isoform v2: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Isoform v1: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Isoform v5: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. lacks enzyme activity. Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Isoform v4: Lacks calcium-dependent NADPH oxidase activity. Isoform v3: Lacks calcium-dependent NADPH oxidase activity. |
| Protein Name | Nadph Oxidase 5 |
| Database Links | Reactome: R-HSA-3299685 |
| Cellular Localisation | Isoform V2: Endoplasmic ReticulumCell MembraneMulti-Pass Membrane ProteinCalcium-Sensitive Association And Dissociation Between The N- And C-Terminal Domains Appears To Facilitate Its Localization To The Cell MembraneIsoform V5: Endoplasmic Reticulum |
| Alternative ELISA Names | Nadph Oxidase 5 ELISA kitNOX5 ELISA kit |
| Specificity | This kit recognizes Human NOX5 in samples. No significant cross-reactivity or interference between Human NOX5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 100 |
| EDTA plasma (n=8) | 88-101 | 93 |
| Cell culture media (n=8) | 87-99 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 95.89 | 220.44 | 938.13 | 97.64 | 229.99 | 959.29 |
| Standard deviation | 4.95 | 8.88 | 48.13 | 6.17 | 12.90 | 39.14 |
| CV (%) | 5.16 | 4.03 | 5.13 | 6.32 | 5.61 | 4.08 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
