• Human Notch 2 protein (Recombinant) (C-His & Avi) {Biotin} (STJP002195)
  • Human Notch 2 protein (Recombinant) (C-His & Avi) {Biotin} (STJP002195)

Human Notch 2 protein (Recombinant) (C-His & Avi) {Biotin} (STJP002195)

SKU:
STJP002195-100

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Host: HEK293 cells
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Notch 2-C-His & Avi protein was developed from hek293 cells and has a target region of C-His & Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOTCH2
Gene ID: 4853
Uniprot ID: NOTC2_HUMAN
Immunogen Region: Leu26-Gln530
Immunogen: Biotinylated Recombinant Human Notch 2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Leu26-Gln530) of Human Notch 2 fused with His tag and Avi tag at the C-terminal.
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Hydroxylated by HIF1AN. Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1. Phosphorylated by GSK3. GSK3-mediated phosphorylation is necessary for NOTCH2 recognition by FBXW7, ubiquitination and degradation via the ubiquitin proteasome pathway.
Function Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells.
Protein Name Neurogenic Locus Notch Homolog Protein 2
Notch 2
Hn2 Cleaved Into - Notch 2 Extracellular Truncation
N2ecd - Notch 2 Intracellular Domain
N2icd
Database Links Reactome: R-HSA-1912399
Reactome: R-HSA-1912408
Reactome: R-HSA-1912420
Reactome: R-HSA-2197563
Reactome: R-HSA-2979096
Reactome: R-HSA-350054
Reactome: R-HSA-5083630
Reactome: R-HSA-9013695
Cellular Localisation Notch 2 Extracellular Truncation: Cell Membrane
Single-Pass Type I Membrane Protein
Notch 2 Intracellular Domain: Nucleus
Cytoplasm
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Retained At The Cytoplasm By Tcim
Alternative Protein Names Neurogenic Locus Notch Homolog Protein 2 protein
Notch 2 protein
Hn2 Cleaved Into - Notch 2 Extracellular Truncation protein
N2ecd - Notch 2 Intracellular Domain protein
N2icd protein
NOTCH2 protein

Information sourced from Uniprot.org

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