Applications: |
ELISA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
0.09ng/mL |
Detection Limit: |
0.16~10ng/mL |
Short Description: |
This NOS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Colormetric |
Gene Symbol: |
NOS1 |
Gene ID: |
4842 |
Uniprot ID: |
NOS1_HUMAN |
Specificity: |
This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Post Translational Modifications | Ubiquitinated.mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). |
Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. |
Protein Name | Nitric Oxide Synthase 1Constitutive NosNc-NosNos Type INeuronal NosN-NosNnosNitric Oxide Synthase - BrainBnosPeptidyl-Cysteine S-Nitrosylase Nos1 |
Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-392154Reactome: R-HSA-5578775 |
Cellular Localisation | Cell MembraneSarcolemmaPeripheral Membrane ProteinCell ProjectionDendritic SpineIn Skeletal MuscleIt Is Localized Beneath The Sarcolemma Of Fast-Twitch Muscle Fiber By Associating With The Dystrophin Glycoprotein ComplexIn NeuronsEnriched In Dendritic Spines |
Alternative ELISA Names | Nitric Oxide Synthase 1 ELISA kitConstitutive Nos ELISA kitNc-Nos ELISA kitNos Type I ELISA kitNeuronal Nos ELISA kitN-Nos ELISA kitNnos ELISA kitNitric Oxide Synthase - Brain ELISA kitBnos ELISA kitPeptidyl-Cysteine S-Nitrosylase Nos1 ELISA kitNOS1 ELISA kit |
Specificity | This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 86-100 | 92 |
EDTA plasma (n=8) | 91-108 | 98 |
Cell culture media (n=8) | 91-106 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 0.50 | 1.40 | 3.50 | 0.50 | 1.30 | 3.40 |
Standard deviation | 0.03 | 0.07 | 0.19 | 0.03 | 0.05 | 0.16 |
CV (%) | 6.00 | 5.00 | 5.43 | 6.00 | 3.85 | 4.71 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance